Catalog Number - 68504-10P-2

Cogent RP C4™ is a reversed-phase HPLC stationary phase designed for the separation of hydrophobic and neutral compounds where less retention is desired than with traditional C8 or C18 phases. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this stationary phase provides reproducible chromatographic performance and is widely used as an alternative in USP methods requiring L26 chemistry. Its reduced hydrophobicity compared to longer-chain bonded phases makes it particularly useful for rapid analyses, method development, and LC-MS workflows.

68504-10P-2 is an HPLC column packed with 5 µm RP C4™ particles featuring a 100 Å pore size in a 2.1 mm ID x 100 mm format. This narrow-bore configuration combines enhanced chromatographic resolution with reduced solvent consumption and excellent compatibility with LC-MS instrumentation. The 100 mm length provides greater resolving power than shorter 50 mm columns while maintaining practical analysis times. The 5 µm particles provide low backpressure and robust operation. Typical operating flow rates are approximately 0.2–0.3 mL/min. As with all 2.1 mm ID columns, the HPLC system must be capable of precise and reproducible low-flow operation.

This column is commonly used for hydrophobic and neutral compounds in reversed-phase HPLC when reduced retention is desirable. Compared to C8 phases, RP C4™ typically provides lower retention, making it useful for compounds that may be excessively retained on more hydrophobic stationary phases. The 2.1 mm ID format is particularly popular for LC-MS workflows due to reduced solvent consumption, improved detector sensitivity, and efficient analyte transfer to the mass spectrometer.

Key Benefits

• RP C4™ stationary phase

• USP L26 equivalent chemistry

• Butyl bonded phase

• Fully double end capped

• Ultra-high purity silica support

• Less retention than C8 phases

• Suitable for rapid analyses

• Lower backpressure than smaller particle columns

• 5 µm particle size

• Excellent for LC-MS applications

Typical Applications

• USP L26 methods

• LC-MS methods

• Method development

• Method scouting

• Hydrophobic compound analysis

• Neutral compound analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• High-throughput analysis

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4™

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for hydrophobic and neutral compounds

• Provides less retention than C8 stationary phases

• Suitable for rapid reversed-phase separations

• The 2.1 mm ID format is ideal for reduced solvent consumption and LC-MS compatibility

• Standard HPLC systems must be capable of reliable operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• The 100 mm length provides greater resolution than 50 mm columns while maintaining practical run times

• 5 µm particles allow lower backpressure operation and robust performance

• Fully double end capped to minimize secondary interactions

• Frequently used for method development and scouting studies